Publications:

Reviews and Background of Covalys' technologies
Papers on labeling using SNAP-tag
Papers on other applications of SNAP-tag

Introductory articles from Covalys
Papers on immobilization using SNAP-tag
Papers on ACP-tag

Reviews and Background of Covalys' technologies
O'Hare et al.: "Chemical probes shed light on protein function." Curr Opin Struct Biol. (2007), 17(4), pp. 488-94. (Review)
Johnsson N. and Johnsson K.: "Chemical Tools for Biomolecular Imaging." ACS Chem Biol. (2007), 2(1), pp. 31-8. (Review)
Johnsson N. et al.: "Protein chemistry on the surface of living cells." Chembiochem. (2005), 6(1), pp. 47-52. (Review)
Gronemeyer T. et al: "Adding value to fusion proteins through covalent labeling", Curr. Oppin. Biotechn. (2006), 16.
Johnsson N. and Johnsson K.: "A fusion of disciplines: chemical approaches to exploit fusion proteins for functional genomics.", Chembiochem (2003), 4(9).
Pegg A. E.: "Repair of O(6)-alkylguanine by alkyl transferases." Mutat. Res. (2000), 462, pp. 83-100
Daniels D. S. and Trainer J. A.: "Conserved structural motifs governing the stoichiometric repair of alkylated DNA by O(6)-alkylguanine-DNA alkyltransferase." Mutat. Res. (2000), 460, pp. 151-163

Introductory articles from Covalys
"Internalization and Protein Relocation: Selective Labeling Approach Can Reduce Intracellular Background", Genetic Engineering & biotechnology News, Vol. 27 No 14 (2007) (347kB)
"Self-labeling protein tags", Bioforum 6/2005, pp.50-51 (399kB)
"Simplified Development of FRET Assays", Drug Discovery Assay Tutorial, Genetic Engineering News, Vol. 25 No 21 (2005) (400kB)

Papers on labeling using SNAP-tag
McMurray MA and Thorner J: "Septin Stability and Recycling during Dynamic Structural Transitions in Cell Division and Development". Current Biology 18, 1-6 August 26.
Howland et al.: "Inducing Efficient Cross-priming Using Antigen-coated Yeast Particles". J. Immunother. 2008, July 2. (Epub ahead of print), PMID: 18600183
Maurel et al.: "Cell-surface protein-protein interaction analysis with time-resolved FRET and SNAP-tag technologies: application to GPCR oligomerization". Nature Methods. (Published online 18 May 2008), DOI: 10.1038/NMETH.1213
Mao et al.: "Optical lock-in detection of fluorescence resonance entergy transfer using synthetic and genetically encoded optical switches". Biophys J BioFAST. (June 2008), Volume 94(11), pp. 4515-24
Banala et al.: "Caged Substrates for Protein Labeling and Immobilization". Chembiochem. (Jan 2008), Volume 4;9(1), pp. 38-41
Pick et al.: "Distribution Plasticity of the Human Estrogen Receptor alpha in Live Cells: Distinct Imaging of Consecutively Expressed Receptors". J Mol Biol. (Dec 2007), Volume 14;374(5), pp. 1213-23
Stenoien D. L. et al.: "Cellular Trafficking of Phospholamban and Formation of Functional Sarcoplasmic Reticulum during Myocyte Differentiation". Am J Physiol Cell Physiol (Feb 7, 2007), Volume 292, pp. C2084-C2094.
Jansen L. et al.: "Propagation of centromeric chromatin requires exit from mitosis". Journal of Cell Biology (March 5, 2007), Volume 176, No. 6, pp. 795-805
Böhme. et al.: "Tracking of human Y receptors in living cells- A fluorescence approach". Peptides (2007), Volume 28, pp. 226-234
Keppler A. et al.: "Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum" BioTechniques (Aug 2006), Volume 41, Nr 2:167-70, 172, 174-5.
Krayl M. et al.:"Fluorescence-mediated analysis of mitochondrial preprotein import in vitro." Anal Biochem 335(1), (Aug 2006); pp. 81-9
Gronemeyer T. et al.:"Directed evolution of O6-alkylguanine-DNA alkyltransferase for applications in protein labeling" Prot Eng Des Sel 19(7), (Jul 2006); pp. 309-16.
Tirat A. et al.: "Evaluation of two novel tag-based labeling technologies for site-specific modification of proteins" Int J Biol Macromol 39(1-3), (Aug 2006); pp. 66-76.
Regoes A. et al.:"SNAP-tag mediated live cell labeling as an alternative to GFP in anaerobic organisms." Bio Techniques (December 2005), Volume 39, Number 6, pp. 809-812
Keppler A. et al.: "Labeling of fusion proteins with synthetic fluorophores in live cells." PNAS 101 (2004), pp. 9955-9959.
Kindermann M. et al.:"Synthesis and characterization of bifunctional probes for the specific labeling o fusion proteins." Bioorg. Med. Chem. Lett 14 (2004), pp. 2725-2728.
Keppler A. et al.:"Labeling of fusion proteins of O(6)-alkylguanine-DNA alkyltransferase with small molecules in vitro and in vivo." Methods 32 (2004), pp. 437-444.
Juillerat A. et al.:"Directed evolution of O(6)-alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo." Chem. Biol. 10 (2003), pp.313-317.
Keppler A. et al.:"A general method for the covalent labeling of fusion proteins with small molecules in vivo." Nature Biotechnology 21 (2003), pp.86-89.

Papers on immobilization with SNAP-tag
Iversen L. et al. :"Templated Protein Assembly on Micro-Contact-Printed Surface Patterns. Use of the SNAP-tag Protein Functionality". Langumuir, 2008 May 17.
Gronemeyer T. et al. :"A new Pull-Down Assay to Monitor Protein-Protein Interactions". Please e-mal support(at)covalys.com for a reprint of the poster.
Jongsma M.A., Litjens R. H.:"Self-assembling protein arrays on DNA chips by auto-labeling fusion proteins with a single DNA address", Proteomics, 2006 May; 6(9), pp. 2650-2655
Sielaff I. et al.:" Protein function microarrays based on self-immobilizing and self-labeling fusion proteins" ChemBioChem Vol 7, Iss 1, pp. 194-202
Kufer S.K. et al.: "Covalent immobilization of recombinant fusion proteins with hAGT for single molecule force spectroscopy.", Eur Biophys J (2005), 35(1), pp. 72-78
Tugulu S. et al.:"Protein-Functionalized Polymer Brushes"  Biomacromolecules 6 (3) (2005), pp.1602-1607.
Huber W. et al.:"SPR-based interaction studies with small molecular weight ligands using hAGT fusion proteins." Analytical Biochemistry 333(2) (2004), pp. 280-288.
Kindermann M. et al.: "Covalent and selective immobilization of fusion proteins." JACS 125(26) (2003), pp. 7810-7811.

Other applications using the SNAP-tag

CLIP-tag Papers

Papers on ACP-tag
Wong et al.: "Directed Site-Selective Covalent Protein Immobilization Catalyzed by a Phosphopantetheinyl Transferase". JACS, publisched online Aug 2008, DOI:10.102/ja8030278
Zhou Z. et al.: "Genetically Encoded Short Peptide Tags for Orthogonal Protein Labeling by Sfp and AcpS Phosphopantetheinyl Transferases" ACS Chemical Biology Vol. 2 (5),(2007) pp. 337-346
Jacquier V. et al.: "Visualizing receptor trafficking in living cells down to the single-molecule level" PNAS Vol. 103 (39), (Sept. 2006), pp.14325-14330
Prummer M. et al.: "Post-translational covalent labeling reveals heterogeneous mobility of individual G protein-coupled receptors in living cells" ChemBioChem 2006, 7, pp. 908-911
Meyer B.H. et al.: "Covalent labeling of cell-surface proteins for in-vivo FRET studies." FEBS Letters, Vol. 580, Iss. 6, pp. 1654-1658
Meyer B.H. et al.:"FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells" Proc Natl Acad Sci U S A. 2006 Feb 14;103(7), pp. 2138-43
Sielaff I. et al.:" Protein function microarrays based on self-immobilizing and self-labeling fusion proteins" ChemBioChem Vol 7, Iss 1, pp. 194-202.
Vivero-Pol L. et al.:"Multicolor imaging of cell surface proteins." J Am Chem Soc. 127 (37) (2005), pp. 12770-12771.
Yin J. et al.: "Single-Cell FRET Imaging of Transferrin Receptor Trafficking Dynamics by Sfp-Catalyzed, Site-Specific Protein Labeling." Chem Biol. 12 (9) (2005), pp. 999-1006.
Cravatt B.F. Comment: "Live Chemical Reports from the Cell Surface." Chem Biol. 12 (9) (2005), pp. 954-956.
George N. et al.: "Specific labeling of cell surface proteins with chemically diverse compounds." J Am Chem Soc. 126 (29) (2004), pp. 8896-8897
Yin J. et al.: "Labeling proteins with small molecules by site-specific posttranslational modification." J Am Chem Soc. 126 (25) (2004), pp. 7754-7755.
La Clair JJ. et al.: "Manipulation of carrier proteins in antibiotic biosynthesis." Chem Biol. 11 (2) (2004), pp. 195-201.