Application Notes & Newsletters

Cell biology applications
NEW! CLIP-tag & SNAP-tag for Simulatneous Protein Labeling Inside Living Cells (2MB)
Wild-type AGT does not give any background when labeling SNAP-fusion proteins in mammalian cells (1.0MB)
Correct localization of SNAP-tag fusion proteins in subcellular organelles (1.8MB)
Flow cytometry analysis of SNAP-tag labeled cells(1.03MB)
SNAP-vitro for selective labeling of cell-surface exposed proteins. (213kB)
Use of SNAP-tag labeling to follow neurokinin-1 receptor internalization induced by Substance P. (1.4MB)
Use of SNAP-tag to follow phorbol ester induced redistribution of Protein Kinase C gamma. (1.2MB)
SNAP-tag fusion proteins localize correctly in cultured mammalian cells. (770kB)
SNAP-vitro for labeling SNAP-tag fusion proteins in fixed CHO-K1 cells. (1.72MB)
SNAP-tag fluorescent labeling is stable to various fixation conditions. (1.1MB)

Protein Interaction Assays
MDM2/p53 interaction assay using SNAP-vitro HTRF® (320kB)
Use of SNAP-vitro HTRF® Donor and Acceptor to develop a FKBP/FRB protein interaction assay
SNAP-vitro for protein interaction assays: FKBP/FRB binding assay using biotin capture on streptavidin coated plates (617kB)

Protein Purification
NEW! SNAP-capture Purification Resin for Efficient Protein Purification from E. coli Lysate (581kB)

Performance and example protocols
SNAP-tag - The multipurpose protein tag (266kB)
SNAP-vista Green and SNAP-vitro 647 for rapid high sensitivity detection of SNAPtag fusion proteins on gels. (1MB)
Characterization of reaction conditions for SNAP-vitro. (179kB)
Use of SNAP-vista to rapidly verify bacterial fusion protein expression. (370kB)
Use of pSET7-26b: Expression of SNAP-tag Cyclophilin A fusion protein in E. coli using pSET7-26b. (220kB)

Newsletters
Newsletter Summer 2008: "Purification of SNAP-tag Fusion Proteins Using SNAP-Capture Purification Resin"
Newsletter Spring 2008: "Use of CLIP-tag and SNAP-tag for Simultaneous Protein Labeling inside Living Cells"
Newsletter Winter 2007: "Protein Dynamics in Living Cells Studied with SNAP-tag"
Newsletter Summer 2007: "SNAP-capture for Protein Immobilization and Pull-Down Applications"
Newsletter Autumn 2007: "SNAP-tag TR-FRET Analysis of MDM2-p53 Interaction"
Newsletter Spring 2007: "SNAP-tag for GPCR Internalization Assays"