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SNAP-vitro HTRF® Donor Kits enable the direct labeling of SNAP-tag fusion proteins with donor fluorophore for HTRF® binding assays. The kits contain the substrate required to label one protein of an interacting pair with BG-TBP (Eu3+ cryptate, HTRF donor; Ex 335nm, long-lifetime Em 580-650nm). SNAP-vitro HTRF® donor labeling is compatible with both SNAP-vitro HTRF acceptor (BG-647) and the standard XL665 (allophycocyanine) acceptor. SNAP-vitro HTRF® Donor kits can be used for labeling SNAP-tag proteins in solution and in cell lysates
HTRF® is a registered trademark of CIS bio international.
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Excitation and emission spectra of HTRF® Donor, BG-TBP, coupled to SNAP-tag in buffer at pH 7.5. |
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SNAP-vitro HTRF® Donor Starter Kit (P/N SK045): 2 single use tubes containing 1 nmol of BG-TBP each, 2 NAP-5 columns for removal of non-reacted substrate, 1 single use tube containing 3 nmol of BG-488 and 1 spin column for removal of non-reacted substrate, for use as a test substrate, and detailed  instructions.
SNAP-vitro HTRF® Donor 5-Kit (P/N LK047): 5 single use tubes containing 1 nmol of BG-TBP each, and detailed instructions.
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