SNAP-vitro HTRF® 647 Acceptor

SNAP-vitro 647 is an excellent FRET acceptor for Eu3+ Cryptate in HTRF® assays. SNAP-vitro 647 is based on BG-647 which features a photostable fluorophore also suitable for use with 650nm laser excitation or appropriate filter sets (Ex 660nm, Em 673nm). SNAP-vitro 647 has many other applications: it can be used for labeling SNAP-tag proteins in solution, or in cell lysates; it can be used for selective labeling of SNAP-tag proteins on cell surfaces and for labeling in fixed permeabilized cells; and it can be used for the rapid specific detection of SNAP-tag fusion proteins directly on SDS-PAGE gels.

Spectra: graph, excitation data, emission data.

Excitation and emission spectra of BG-647 coupled to SNAP-tag at pH 7.5.

Kits:

SNAP-vitro 647 Starter Kit (P/N SK043): 5 single use tubes containing 3 nmol of BG-647 each, 5 spin columns for removal of non-reacted substrate, 500nmol of SNAP-block for control experiments, DMSO to dissolve SNAP-block, and detailed instructions.

SNAP-vitro 647 100-Kit (P/N LK044): 1 tube containing 100 nmol of BG-647, DMSO to dissolve substrate, and detailed instructions.

Literature:

Relevant application notes:
MDM2/p53 interaction assay using SNAP-vitro HTRF®
Use of SNAP-vitro HTRF® Donor and Acceptor to develop a FKBP/FRB protein interaction assay
SNAP-vitro for protein interaction assays: FKBP/FRB binding assay using biotin capture on streptavidin coated plates
Characterization of reaction conditions for SNAP-vitro
SNAP-vista Green and SNAP-vitro 647 for rapid high sensitivity detection of SNAP-tag fusion proteins on gels
SNAP-vitro for selective labeling of cell-surface exposed proteins
SNAP-vitro for labeling SNAP-tag fusion proteins in fixed CHO-K1 cells
Flow cytometry analysis of SNAP-tag labeled cells

Relevant publications:
"Simplified Development of FRET Assays", Drug Discovery Assay Tutorial, Genetic Engineering News, Vol. 25 No 21 (2005)

All application notes All publications