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SNAP-vista for protein detection in gels | | SNAP-vista products provide a very convenient and simple alternative to performing a Western blot. They allow the specific detection of SNAP-tag fusion proteins directly on an SDS-PAGE gel.
SNAP-tag fusion proteins present in samples (even in crude lysates) are specifically labeled in a 20 minute, single reagent reaction prior to loading the gel. The fluorescently labeled protein is detected in the gel by UV-transillumination (SNAP-vista Blue, SNAP-vista Green) or by a gel scanning system with 488nm or 633/650nm excitation (SNAP-vista Green, BG-647 respectively). |
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Product | Comment | SNAP-vista Blue | For detection on a UV-transilluminator, sensitivity ≤100 ng/band (Ex 390nm, Em 470nm) | SNAP-vista Green | For detection on a UV-transilluminator, sensitivity ≤100 ng/band, and on suitable gel scanners with 488nm excitation, sensitivity ≤20 pg per band (Ex 500nm, Em 524nm) | SNAP-vitro 647 | For use on suitable gel scanners, sensitivity ≤20 pg per band (Ex 660nm, Em 673nm) |
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 Datasheet
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