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| | Transient expression of SNAP-actin labeled with BG-430, BG-505, BG-DAF or TMR-Star (CHO-K1 cells) |
SNAP-cell products contain cell-permeable sustrates for rapid precision labeling of SNAP-tag fusion proteins inside live cells. It is ideal for protein localization experiments, protein translocation, flow cytometry studies, and more. In addition, certain SNAP-vitro substrates, which are not cell-permeable, are particularly suited to exclusively label extracellular domains of membrane proteins. This labeling approach can be used for example to follow receptor internalization. SNAP-tag labeling has worked well in all mammalian cell lines tested so far including CHO-K1, COS-7, HEK 293, HeLa, NIH 3T3, V79, and U-2 OS. The SNAP-tag is based on the DNA repair protein AGT, but because it is highly optimized for reaction with SNAP-tag substrates, no background labeling from wtAGT is seen ( further information).
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For labeling inside live cells | Product | Comment | SNAP-cell Trial Kit
| For rapidly evaluating SNAP-tag labeling with novel cell lines and imaging technologies | SNAP-cell
360 | Suitable for DAPI filter sets, Ex 357 nm, Em 437 nm | SNAP-cell
430 | Bright blue fluorophore, Ex 421 nm, Em 444/484 nm | SNAP-cell
505 | For fluorescein filter set, more photostable than fluorescein, Ex 504 nm, Em 532 nm | SNAP-cell
DAF | Fluorescein as fluorophore, Ex 500 nm, Em 524 nm | SNAP-cell TMR-Star | Suitable for Rhodamine filter sets, Ex 554 nm, Em 580 nm | SNAP-block Cell | Blocks all labeling of SNAP-tag in live cells. Useful to generate controls and as a blocker for pulse-chase. | For cell-surface selective labeling | Product | Comment | SNAP-cell Surface Trial Kit | For rapidly evaluating SNAP-tag cell-surface selective labeling with novel cell lines and imaging technologies | SNAP-Alexa Fluor® 488 | For fluorescein filter sets, Ex 496 nm, Em 520 nm | SNAP-vitro 488 | For fluorescein filter sets, Ex 506 nm, Em 526 nm | SNAP-Alexa Fluor® 546 | For TAMRA and Cy3 filter sets, Ex 558 nm, Em 574 nm | SNAP-vitro 547 | For TAMRA and Cy3 filter sets, Ex 554 nm, Em 568 nm | SNAP-Alexa Fluor® 647 | For 650 nm laser line, Ex 652 nm, Em 670 nm | SNAP-vitro 647 | For 650 nm laser line, Ex 660 nm, Em 673 nm | SNAP-SureLight® Allophycocyanin | For dedicated Allophycocyanin filter sets, Ex 652 nm, Em 666 nm | SNAP-block Cell Surface
Datasheet
| Selectively blocks labeling of SNAP-tag exposed on cell surface. Useful to generate controls and as a blocker for pulse-chase. |
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