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| | Transient expression of CLIP-tag fusion proteins labeled with CT-430, CT-TMR, CT-360 or CT-505 (CHO-K1 cells) |
CLIP-cell products contain cell-permeable sustrates for specific labeling of CLIP-tag fusion proteins inside live cells.
CLIP-tag is a small protein tag based on the DNA repair protein AGT. CLIP-tag substrates are derivates of benzylcytosine (CT). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag, forming a stable thioether link. Although CLIP-tag is based on the same protein as SNAP-tag, the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag can therefore be used for orthogonal simultaneous labeling.
CLIP-cell substrates are ideal for dual protein labeling, protein localization experiments, protein dynamics and protein translocation, flow cytometry studies, and more. In addition, CLIP-vitro substrates, which are not cell-permeable, are particularly suited to exclusively label extracellular domains of membrane proteins. This labeling approach can be used for example to follow receptor internalization. CLIP-tag labeling has worked well in all mammalian cell lines tested so far. |
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For labeling inside live cells | Product | Comment | CLIP-cell Trial Kit
| For rapidly evaluating CLIP-tag labeling with novel cell lines and imaging technologies | CLIP-cell
360 | Suitable for DAPI filter sets, Ex 357 nm, Em 437 nm | CLIP-cell
430 | Bright blue fluorophore, Ex 421 nm, Em 444/484 nm | CLIP-cell
505 | For fluorescein filter set, more photostable than fluorescein, Ex 504 nm, Em 532 nm | CLIP-cell
PF | Cell permeable fluorescein ester, Ex 500 nm, Em 524 nm | CLIP-cell TMR | Suitable for Rhodamine filter sets, Ex 554 nm, Em 580 nm | CLIP-cell Block | Blocks all existing CLIP-tag fusion proteins in live cells. Useful to generate controls and as a blocker for pulse-chase. | For cell-surface selective labeling | Product | Comment | CLIP-cell Surface Trial Kit | For rapidly evaluating CLIP-tag cell-surface selective labeling with novel cell lines and imaging technologies | CLIP-vitro 488 | For fluorescein filter sets, Ex 506 nm, Em 526 nm | CLIP-vitro 547 | For TAMRA and Cy3 filter sets, Ex 554 nm, Em 568 nm | CLIP-vitro 647 | For 650 nm laser line, Ex 660 nm, Em 673 nm |  Datasheet
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