ACPwt-tag is based on the Acyl Carrier Protein from E. coli, and can be labeled with Coenzyme A derivatives using ACPwt-Synthase or SFP-Synthase. MCP-tag is a mutant version of the Acyl Carrier Protein (D36-T36 & D39-G39) and is preferentially covalently labeled with SFP-Synthase. The Coenzyme A Substrates can be used to label both tags.
ACPwt-tag and MCP-tag products include a variety of CoA-based labeling substrates, the labeling enzyme ACPwt-Snythase and the SFP-Synthase to label ACPwt-tag or ACPwt-tag and MCP-tag respectively. Both expression and source plasmids are available to express the tags. The small ACPwt-tag and MCP-tag (both 77 aa) are particularly suited to selectively label the extracellular portion of membrane proteins. In addition, having no cysteines, they should be useful for labeling secreted proteins with disulphide bridges such as antibodies. The SFP-Synthase is also compatible with labeling PCPwt and shorter versions thereof.
