Product Overview

Covalys offers a broad range of kits for expression of tagged fusion proteins and for the subsequent stable modification of these proteins. All kits contain substrates or immobilization surfaces, supplementary materials and comprehensive instructions for use, helping you to start your experiments straight away.

Kits include a free license for use of the materials.

For all Covalys protein tags we have a significant range of substrates available which are not yet introduced as standard products. If you should be interested in something not yet offered here, please get in touch with us. We are happy to discuss your application and offer the corresponding material as far as available. Depending on your needs we can also offer you custom synthesis of your particular substrates. Please do not hesitate to contact us!

SNAP-tag products

SNAP-tag is a protein tag of
20 kDa and specifically reacts with its benzylguanine (BG) substrates, thereby forming a covalent, thus highly stable thioether bond. SNAP-tag substrates are chemically stable and are not reactive to other proteins. Applications range from intracellular and cell surface labeling, over simplified detection on an SDS-PAGE gel, to kits for pull down assays and purification of fusion proteins.

CLIP-tag products

CLIP-tag is a sibling of the SNAP-tag of identical size, but reacting with benzylcytosine (CT) derivatives which form a separate class of substrates. Applications so far include intracellular and cell surface labeling, and simplified detection of CLIP-tag proteins on an SDS-PAGE gel.

ACP/MCP-tag products

ACP-tag and MCP-tag are small protein tags with about 8 kDa molecular weight, suitable for cell surface protein labeling only. ACP-tag and MCP-tag allow sequential dual color labeling of two different proteins. The reduced size of these tags may be beneficial for certain applications. The presence of a particular synthase is required for the formation of a covalent link between the ACP-tag fusion protein and the Coenzyme A based substrate. Due to the use of these charged substrates in combination with the synthase, the application of the ACP-tag and the MCP-tag are therefore restricted to cell surface proteins and cell free systems.