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How it works:
SNAP-tag is fused to the extracellular part of the target receptor. The fusion protein is expressed in an appropriate cell line. Non-cell permeable SNAP-tag substrate is used for selective labeling of receptors exposed on the cell membrane. Upon stimulation, internalization is followed by fluoresence microscopy.
Advantages:
- Clear signal as there is no background from internal receptor pool.
- Fluorescent label can be selected to best fit instrumentation.
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| Resting | | Stimulated | Internalization of SNAP-Neurokinin 1 receptor labeled with BG-488 (green) upon stimulation with Substance P in COS-7 cells, counterstained with Hoechst 33342 (blue). |
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