Novel applications of SNAP-tag


Extending the boundaries


The world of SNAP-tag applications is constantly expanding. Recent examples include:
- purification of SNAP-tag fusion proteins
- single molecule force spectroscopy
- pulse-chase imaging
- live-cell imaging in anaerobes
- whole-organ imaging in Drosophila embryos
- functional protein arrays
- DNA-oligo encoded functional protein arrays
We are always happy to collaborate with scientists interested in new applications. Just let us know what you want to do with Covalys' technology!

Pulse-chase experiment: SNAP-actin transiently expressed in NIH 3T3 cells was labeled 24h post transfection with TMR-Star (red; "pulse") and 24h later with BG-505 (green; "chase"). Left, whole cell; right: insert (50 micrometers high); arrow: region with mainly freshly synthesized actin. Imaged on Zeiss CLSM 510.
Courtesy Teapal, Lankenau, IBMT Berlin.

 
Products:

Cell permeable CLIP-tag substrates

CLIP-cell 360

CLIP-cell 430

CLIP-cell 505

CLIP-cell PF

CLIP-cell TMR

CLIP-cell Trial Kit

Cell permeable SNAP-tag substrates

SNAP-cell 360

SNAP-cell 430

SNAP-cell 505

SNAP-cell DAF

SNAP-cell TMR-Star

SNAP-cell Trial Kit

SNAP-tag Building Blocks allow you to turn your label or surface into a SNAP-tag substrate. 
Or let us know your intended application, we may have the solution on hand or in development.

 

Literature:

Publications:

Pick et al.: "Distribution Plasticity of the Human Estrogen Receptor alpha in Live Cells: Distinct Imaging of Consecutively Expressed Receptors". J Mol Biol. (Dec 2007), Volume 14;374(5), pp. 1213-23
Stenoien D. L. et al.: "Cellular Trafficking of Phospholamban and Formation of Functional Sarcoplasmic Reticulum during Myocyte Differentiation". Am J Physiol Cell Physiol (Feb 7, 2007), Volume 292, pp. C2084-C2094.
Jansen L. et al.: "Propagation of centromeric chromatin requires exit from mitosis". Journal of Cell Biology (March 5, 2007), Volume 176, No. 6, pp. 795-805
Jongsma M.A., Litjens R. H.:"Self-assembling protein arrays on DNA chips by auto-labeling fusion proteins with a single DNA address", Proteomics, 2006 May; 6(9), pp. 2650-2655
Regoes A. et al.:"SNAP-tag mediated live cell labeling as an alternative to GFP in anaerobic organisms." Bio Techniques (December 2005), Volume 39, Number 6, pp. 809-812
Sielaff I. et al:" Protein function microarrays based on self-immobilizing and self-labeling fusion proteins" ChemBioChem 2005, Vol 7, Iss 1, pp. 194-202
Kufer S.K. et al: "Covalent immobilization of recombinant fusion proteins with hAGT for single molecule force spectroscopy.", Eur Biophys J (2005), 35(1), pp. 72-78
Tugulu S. et al.:"Protein-Functionalized Polymer Brushes"  Biomacromolecules 6 (3) (2005), pp.1602-1607.

Newsletter Winter 2007: "Protein Dynamics in Living Cells Studied with SNAP-tag"