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» Overview
Receptor Internalization
Selective cell-surface labeling
Protein localization
Protein translocation
CLIP-tag & SNAP-tag for simultaneous protein labeling inside living cells
Flow-cytometry
Binding assays in microtiter plates
FRET-based binding assays
Protein purification
Biosensor interaction experiments
Protein detection
Pulse Chase & further applications
Application notes overwiew

Protein translocation


Detect stimulation of signaling pathways by following translocation of reporter proteins.


How it works:
The protein of interest is expressed as a SNAP-tag fusion in the host cell and labeled using cell-permeable SNAP-cell substrates. Translocation upon stimulation is followed by fluorescent microscopy.
Advantages:
- Easy change of fluorophore.
- License for commercial use included.

Resting

Stimulated

Translocation of TMR-Star labeled Protein Kinase C gamma-SNAP induced by phorbol ester treatment in U-2 OS cells.
 
Products:

SNAP-cell 430

SNAP-cell 505

SNAP-cell DAF

SNAP-cell TMR-Star

 

 

 
Literature:

Application notes:
 Use of SNAP-tag to follow phorbol ester induced redistribution of Protein Kinase C gamma

Publications:
 Keppler A. et al.:"Labeling of fusion proteins of O(6)-alkylguanine-DNA alkyltransferase with small molecules in vitro and in vivo." Methods 32 (2004), pp. 437-444.