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How it works:
Sepharose beads (BG-Sepharose) modifided with SNAP-tag substrate benzylguanine covalently immobilize SNAP-tag fusion proteins from lysates to its surface. Due to the very stable covalent bond, the beads can be washed using very stringent washing conditions and the protein of interest can be harvested by protease cleavage.
Advantages:
- High binding capacity of SNAP-tag fusion proteins, making it suitable for small and large scale immobilization of proteins followed by purification.
- Specific covalent immobilization from lysate - very stingent washing conditions may be chosen.
- Labeling occurs via SNAP-tag - activity of protein of interest is preserved.
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