FRET-based binding assays

Detect protein-protein interaction in a mix and read assay

How it works:
Two interacting SNAP-tag fusion proteins are separately labeled in cell lysate: one  one with a long lived FRET donor, one with the acceptor. Protein binding is measured by time-resolved fluorescence resonance energy transfer.
Advantages:
- Specific covalent labeling in lysate, no prior purification needed.
- One label per protein, no need to determine labeling stoichiometry.
- Labeling is directed so protein is free to interact.