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How it works:
A SNAP-tag fusion to the protein of interest is expressed in the host cell and labeled using cell-permeable SNAP-cell substrates (intracellularly expressed proteins) or cell impermeable SNAP-tag substrate (proteins exposed on the cell membrane or fixed cells). Cells are counted or sorted using flow cytometry.
Advantages:
- Rapid specific labeling protocol, no antibodies required.
- Easy exchange of wide range of fluorophores.
- Live cell intracellular fluorescent labeling.
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| Flow cytometric analysis of non-transfected CHO-K1 cells (left) or CHO-K1 cells transiently expressing Histone 2B-SNAP (right) labeled with BG-505.
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