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How it works:
SNAP-tag specifically recoginizes substrates which are based on benzyl guanine, wherease CLIP-tag - a close relative of SNAP-tag, specifically reacts with bnezylcytosine derivates, which form a different class of substrates. Thus the two tags can be used to label two different proteins in the same cell, which offers researchers a unique tool to expolore protein dynamics in living cells.
Simultaneous use of CLIP-tag and SNAP-tag allow visualization of two different fusion proteins inside living cells, in real time and under biological conditions. Proteins can be lableed in at time resolved manner which further contributes to the potential of the technology.
Advantages:
- Visualize two different proteins in one single cell in a time resolved manner.
- License for commercial use included.
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| Figure 1 | | Figure 2 | Figure 1: In vivo protein co-labeling with the orthogonal CLIP-tag / SNAP-tag system. Benzylguanine (BG) and benzylcytosine (CT) based fluorescent substrates added to the culture medium react with the proteins of interest fused to the SNAP-tag or CLIP-tag respectively
Figure 2: Live cell fluorescent imaging of the protein kinase MEK1 and histone H2B tagged with CLIP-tag and SNAP-tag, respectively. CHO-K1 cells, expressing one (A) or two (B) of the fusion proteins, were incubated with the cell permeable substrate BG-505 (green) and CT-TMR (red), washed, counterstained with Hoechst and imaged. |
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